Hauptinhalt

Publications:

Wang T, Shao J, Kumar S, Alnouri MW, Carvalho J, Günther S, Krasel C, Murphy KT, Bünemann M, Offermanns S, Wettschureck N. Orphan GPCR GPRC5C facilitates angiotensin II-induced smooth muscle contraction. Circ Res. 2024 Apr 10. doi: 10.1161/CIRCRESAHA.123.323752.

Kurz M, Ulrich M, Bittner A, Bünemann M. DP2 receptor activity sensor suited for antagonist screening and measurement of receptor dynamics in real-time. Sci Rep. 2024 14(1):8178. doi: 10.1038/s41598-024-58410-2.

Kirchhofer S, Lim VJY, Ernst S, Karsai N, Julia RG, Canals M, Kolb P, Bünemann M. Differential interaction patterns of opioid analgesics with µ opioid receptors correlate with ligand-specific voltage sensitivity. eLife 2023 20;12:e91291. dot: 10.7554/eLife.91291.

Kurz M, Ulrich M, Bittner A, Scharf MM, Shao J, Wallenstein I, Lemoine H, Wettschureck N, Kolb P, Bünemann. EP4 receptor conformation sensor suited for ligand screening and imaging of extracellular prostaglandins. Mol Pharmacol. 2023 104(3):80-91. 

Redlin F, Krett AL, Bünemann M. Interaction kinetics between p115-RhoGEF and Gα13 are determined by unique molecular interactions affecting agonist sensitivity. Commun Biol. 2022 5(1):1287. doi: 10.1038/s42003-022-04224-9.

Al-Zaid B, Chacko S, Ezeamuzie CI, Bünemann M, Krasel C, Karimian T, Lanzerstorfer P, Al-Sabah S. Differential effects of glucose-dependent insulinotropic polypeptide receptor/glucagon-like peptide-1 receptor heteromerization on cell signalling when expressed in HEK-293 cells. Pharmacol Res Perspect. 2022 10(5):e01013. doi: 10.1002/prp2.1013

Hedderich JB, Persechino M, Becker K, Heydenreich FM, Gutermuth T, Bouvier M, Bünemann M, Kolb P. The pocketome of G-protein-coupled receptors reveals previously untargeted allosteric sites. Nat Commun. 2022 13(1):2567. doi: 10.1038/s41467-022-29609-6

Mösslein N, Pohle LG, Fuss A, Bünemann M, Krasel C. Residency time of agonists does not affect the stability of GPCR-arrestin complexes. Br J Pharmacol. 2022 179(16):4107-4116. doi: 10.1111/bph.15846

Flöser A, Becker K, Kostenis E, König G, Krasel C, Kolb P, Bünemann M. Disentangling bias between G_q, GRK2, and arrestin3 recruitment to the M₃ muscarinic acetylcholine receptor. Elife 2021 10:e58442. doi: 10.7554/eLife.58442

Jelinek V, Mösslein N, Bünemann M. Structures in G proteins important for subtype selective receptor binding and subsequent activation. Commun Biol 2021 4(1):635. doi: 10.1038/s42003-021-02143-9

Isbilir A, Duan Sahbaz B, Tuncgenc G, Bünemann M, Lohse MJ, Birgül-lyison N. Pharmacological characterization of the stick insect Carausius morosus allatostatin-C receptor with its endogenous agonist. ACS Omega 2020 5(50):32183-32194. doi: 10.1021/acsomega.0c03382

Al-Sabah S, Adi L, Bünemann M, Krasel C. The effect of cell surface expression and linker sequence on the recruitment of arrestin to the GIP receptor. Front Pharmacol. 2020 13;11:1271. doi: 10.3389/fphar.2020.01271

Ruland JG, Kirchhofer S, Klindert S, Bailey CP, Bünemann M. Voltage modulates the effect of µ receptor activation in a ligand-dependent manner. Br J Pharmacol. 2020 177(15):3489-3504. doi: 10.1111/bph.15070

Kurz M, Krett AL, Bünemann M. Voltage dependence of prostanoid receptors. Mol Pharmacol. 2020 97(4):267-277. doi: 10.1124/mol.119.118372

Carvalho J, Chennupati R, Li R, Günther S, Kaur H, Zhao W, Tonak S, Kurz M, Mößlein N, Bünemann M, Offermanns S, Wettschureck N. Orphan G protein-coupled receptor GPRC5B controls smooth muscle contractility and differentiation by inhibiting prostacyclin receptor signaling. Circulation 2020 141(14):1168-1183. doi: 10.1161/CIRCULATIONAHA.119.043703

Scharf MM, Bünemann M, Baker JG, Kolb P. Comparative docking to distinct G protein-coupled receptor conformations exclusively yields ligands with agonist efficacy. Mol Pharmacol. 2019 96(6):851-861. doi: 10.1124/mol.119.117515

Maimari T, Krasel C, Bünemann M, Lorenz K. The N-termini of GRK2 and GRK3 simulate the stimulating effects of RKIP on ß-adrenoceptors. Biochem Biophys Res Commun. 2019 520(2):327-332. doi: 10.1016/j.bbrc.2019.09.135

Annala S, Feng X, Shridhar N, Eryilmaz F, Patt J, Yang J, Pfeil EM, Cervantes-Villagrana RD, Inoue A, Häberlein F, Slodczyk T, Reher R, Kehraus S, Monteleone S, Schrage R, Heycke N, Rick U, Engel S, Pfeifer A, Kolb P, König G, Bünemann M, Tüting T, Vázquez-Prado J, Gutkind JS, Gaffal E, Kostenis E. Direct targeting of Gα_q and Gα₁₁ oncoproteins in cancer cells. Sci Signal. 2019 Mar 19;12(573). pii: eaau5948. doi: 10.1126/scisignal.aau5948

Mattern A, Machka F, Wickleder MS, Ilyaskina OS, Bünemann M, Diener M, Pouokam E. Potentiation of the activation of cholinergic receptors by multivalent presentation of ligands supported on gold nanoparticles. Org Biomol Chem. 2018 16(36):6680-6687. doi: 10.1039/c8ob01686k

Miess E, Gondin AB, Yousuf A, Steinborn R, Mösslein N, Yang Y, Göldner M, Ruland JG, Bünemann M, Krasel C, Christie MJ, Halls ML, Schulz S, Canals M. Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization. Sci Signal. 2018 Jul 17;11(539). pii: eaas9609. doi: 10.1126/scisignal.aas9609.

Ilyaskina OS, Lemoine H, Bünemann M. Lifetime of muscarinic receptor-G-protein complexes determines coupling efficiency and G-protein subtype selectivity. Proc Natl Acad Sci USA. 2018 115(19):5016-5021. doi: 10.1073/pnas.1715751115

Andressen KW, Ulsund AH, Krobert KA, Lohse MJ, Bünemann M, Levy FO. Related GPCRs couple differently to G_s: preassociation between G protein and 5-HT₇ serotonin receptor reveals movement of Gα_s upon receptor activation. FASEB J. 2018 32(2):1059-1069. doi: 10.1096/fj.201700486R

Hoppe A, Marti-Solano M, Drabek M, Bünemann M, Kolb P, Rinne A. The allosteric site regulates the voltage sensitivity of muscarinic receptors. Cell Signal. 2018 42:114-126. doi: 10.1016/j.cellsig.2017.10.011

Dembla S, Behrendt M, Mohr F, Goecke C, Sondermann J, Schneider FM, Schmidt M, Stab J, Enzeroth R, Leitner MG, Nuñez-Badinez P, Schwenk J, Nürnberg B, Cohen A, Philipp SE, Greffrath W, Bünemann M, Oliver D, Zakharian E, Schmidt M, Oberwinkler J. Anti-nociceptive action of peripheral µ-opioid receptors by Gßγ protein-mediated inhibition of TRPM3 channels. Elife. 2017 Aug 15;6. pii: e26280. doi: 10.7554/eLife.26280

Bodmann EL, Krett AL, Bünemann M. Potentiation of receptor responses induced by prolonged binding of Gα13 and leukemia-associated RhoGEF. FASEB J. 2017 31(8):3663-3676. doi: 10.1096/fj.201700026R

Honrath B, Matschke L, Meyer T, Magerhans L, Perocchi F, Ganjam GK, Zischka H, Krasel C, Gerding A, Bakker BM, Bünemann M, Strack S, Decher N, Culmsee C, Dolga AM. SK2 channels regulate mitochondrial respiration and mitochondrial Ca²⁺ uptake. Cell Death Differ. 2017 24(5):761-773. doi: 10.1038/cdd.2017.2

Sun T, Yang L, Kaur H, Pestel J, Looso M, Nolte H, Krasel C, Heil D, Krishnan RK, Santoni MJ, Borg JP, Bünemann M, Offermann S, Swiercz JM, Worzfeld T. A reverse signaling pathway downstream of Sema4A controls cell migration via Scrib. J Cell Biol. 2017 216(1): 199-215. doi: 10.1083/jcb.201602002

Zindel D, Engel S, Bottrill AR, Pin JP, Prézeau L, Tobin AB, Bünemann M, Krasel C, Buchter AJ. Identification of key phosphorylation sites in PTH1R that determine arrestin-3 binding and fine-tune receptor signaling. Biochem J. 2016 473(22):4173-4192. doi:10.1042/BCJ20160740 

Rinne A, Mobarec JC, Mahaut-Smith M, Kolb P, Bünemann M. The mode of agonist binding to a G protein-coupled receptor switches the effect that voltage changes have on signaling. Sci Signal. 2015 Nov 3; 8(401):ra110. doi: 10.1126/scisignal.aac7419

Birk A, Rinne A, Bünemann M. Membrane potential controls the efficacy of catecholamine-induced ß1-adrenoceptor activity. J Biol Chem. 2015 290(45):27311-27320. doi: 10.1074/jbc.M115.665000

Bodmann EL, Wolters V, Bünemann M. Dynamics of G protein effector interactions and their impact on timing and sensitivity of G protein-mediated signal transduction. Eur J Cell Biol. 2015 94(7-9):415-419. doi: 10.1016/j.ejcb.2015.06.004

Reddy GR, Subramanian H, Birk A, Milde M, Nikolaev VO, Bünemann M. Adenylyl cyclases 5 and 6 underlie PIP3-dependent regulation. FASEB J. 2015 29(8):3458-3471. doi: 10.1096/fj.14-268466

Ahles A, Rodewald F, Rochais F, Bünemann M, Engelhardt S. Interhelical interaction and receptor phosphorylation regulate the activation kinetics of different human ß1-adrenoceptor variants. J Biol Chem. 2015 290(3):1760-1769. doi: 10.1074/jbc.M114.607333

Zindel D, Butcher AJ, AL-Sabah S, Lanzerstorfer P, Weghuber J, Tobin AB, Bünemann M, Krasel C. Engineered hyperphosphorylation of the ß2-adrenoceptor prolongs arrestin-3 binding and induces arrestin internalization. Mol Pharmacol. 2015 87(2):349-362. doi: 10.1124/mol.114.095422

Wilke BU, Lindner M, Greifenberg L, Albus A, Kronimus Y, Bünemann M, Leitner MG, Oliver D. Diacylglycerol mediates regulation of TASK potassium channels by Gq-coupled receptors. Nat Commun. 2014 Nov 25;5:5540. doi: 10.1038/ncomms6540

Wolters V, Krasel C, Brockmann J, Bünemann M. Influence of gαq on the dynamics of m3-acetylcholine receptor-g-protein-coupled receptor kinase interaction. Mol Pharmacol. 2015 87(1):9-17. doi: 10.1124/mol.114.094722

Al-Sabah S, Al-Fulaij M, Shaaban G, Ahmed HA, Mann RJ, Donnelly D, Bünemann M, Krasel C. The GIP receptor displays higher basal activity than the GLP-1 receptor but does not recruit GRK2 or arrestin3 effectively. PLoS One 2014 9(9):e106890. doi: 10.1371/journal.pone.0106890

Bodmann EL, Rinne A, Brandt D, Lutz S, Wieland T, Grosse R, Bünemann M. Dynamics of Gαq-protein-p63RhoGEF interaction and its regulation by RGS2. Biochem J. 2014 458(1):131-140. doi: 10.1042/BJ20130782

Milde M, Werthmann RC, von Hayn K, Bünemann M. Dynamics of adenylate cyclase regulation via heterotrimeric G-proteins. Biochemical Soc Trans 2014 42(2):239-243. doi: 10.1042/BST20130280

Milde M, Rinne A, Wunder F, Engelhardt S, Bünemann M. Dynamics of Gα1 interaction with type 5 adenylate cyclase reveal the molecular basis for high sensitivity of Gi-mediated inhibition of cAMP production. Biochem J. 2013 454(3):515-523. doi: 10.1042/BJ20130554

Rinne A, Birk A, Bünemann M. Voltage regulates adrenergic receptor function. Proc Natl Acad Sci USA 2013 110(4):1536-1541. doi: 10.1073/pnas.1212656110

Hoffmann C, Nuber S, Zabel U, Ziegler N, Winkler C, Hein P, Berlot CH, Bünemann M, Lohse MJ. Comparison of the activation kinetics of the M3 acetylcholine receptor and a constitutively active mutant receptor in living cells. Mol Pharmacol. 2012 82(2):236-245. doi: 10.1124/mol.112.077578

Ahles A, Rochais F, Frambach T, Bünemann M, Engelhardt S. A polymorphism-specific "memory" mechanism in the ß(2)-adrenergic receptor. Sci Signal. 2011 4(185):ra53. doi: 10.1126/scisignal.2001681

Werthmann RC, Lohse MJ, Bünemann M. Temporally resolved cAMP monitoring in endothelial cells uncovers a thrombin-induced [cAMP] elevation mediated via the Ca²⁺-dependent production of prostacyclin. J Physiol. 2011 589(1):181-193. doi: 10.1113/jphysiol.2010.200121

Hommers LG, Klenk C, Dees C, Bünemann M. G proteins in reverse mode - receptor-mediated GTP release inhibits G protein and effector function. J Biol Chem. 2010 285(11):8227-8233. doi: 10.1074/jbc.M109.015388

von Hayn K, Werthmann RC, Nikolaev VO, Hommers LG, Lohse MJ, Bünemann M. Gq-mediated Ca²⁺ signals inhibit adenylyl cyclases 5/6 in vascular smooth muscle cells. Am J Physiol Cell Physiol. 2010 298(2):C324-32. doi: 10.1152/ajpcell.00197.2009

Baumer Y, Spindler V, Werthmann RC, Bünemann M, Waschke J. Role of Rac 1 and cAMP in endothelial barrier stabilization and thrombin-induced barrier breakdown. J Cell Physiol. 2009 220:716-726. doi: 10.1002/jcp.21819

Werthmann RC, von Hayn K, Nikolaev VO, Lohse MJ, Bünemann M. Real-time monitoring of cAMP levels in living endothelial cells: thrombin transiently inhibits adenylyl cyclase 6. J Physiol. 2009 587:4091-4104. doi: 10.1113/jphysiol.2009.172957

Dorsch S, Klotz KN, Engelhardt S, Lohse MJ, Bünemann M. Analysis of receptor oligomerization by FRAP microscopy. Nature Methods 2009 3:225-230. doi: 10.1038/nmeth.1304

Hartmann M, Skryabin BV, Müller T, Gazinski A, Schröter J, Gassner B, Nikolaev VO, Bünemann M, Kuhn M. Alternative splicing of the guanylyl cyclase-A receptor modulates atrial natriuretic peptide signaling. J Biol Chem. 2008 283(42):28313-28320. doi: 10.1074/jbc.M805521200

Nikolaev VO, Boettcher C, Dees C, Bünemann M, Lohse MJ, Zenk MH. Live cell monitoring of µ-opioid receptor-mediated G-protein activation reveals strong biological activity of close morphine biosynthetic precursors. J Biol Chem. 2007 282(37):27126-27132. doi: 10.1074/jbc.M703272200

Hullin R, Matthes J, von Vietinghoff S, Bodi I, Rubio M, D'Souza K, Friedrich Khan I, Rottlander D, Hoppe UC, Mohacsi P, Schmitteckert E, Gilsbach R, Bünemann M, Hein L, Schwartz A, Herzig S. Increased expression of the auxiliary ß(2)-subunit of ventricular L-type Ca²⁺ channels leads to single-channel activity characteristic of heart failure. PLoS One 2007 2(3):e292. doi: 10.1371/journal.pone.0000292

Rochais F, Vilardaga J-P, Viacheslav O Nikolaev, Bünemann M,  Lohse MJ, Engelhardt S. Real-time optical recording of β1- adrenergic receptor activation and signaling reveals supersensitivity of the Arg-389 variant to carvedilol. J Clin Invest. 2007 117(1):229-235. doi: 10.1172/JCI30012

Hein P, Rochais F, Hoffmann C, Dorsch S, Nikolaev VO, Engelhardt S, Berlot CH, Lohse MJ, Bünemann M. Gs activation is time-limiting in initiating receptor-mediated signaling. J Biol Chem. 2006 281:33345-33351. doi: 10.1074/jbc.M606713200

Nikolaev VO, Bünemann M, Lohse MJ, Engelhardt S. Cyclic AMP imaging in adult cardiac myocytes reveals far-reaching b1-adrenergic but locally confined b2-adrenergic receptor-mediated signaling. Circ Res. 2006 99:1084-1089. doi: 10.1161/01.RES.0000250046.69918

Nikolaev VO, Hoffmann C, Bünemann M, Lohse MJ, Vilardaga JP. Molecular basis of partial agonism at the neurotransmitter α 2A-adrenergic receptor and Gi-protein heterotrimer. J Biol Chem. 2006 281:24506-24511. doi: 10.1074/jbc.M603266200

Makino A, Prossnitz ER, Bünemann M, Wang JM, Yao W, Schmid-Schonbein GW. G Protein-coupled receptors serve as mechanosensors for fluid shear stress in neutrophils. Am J Physiol Cell Physiol. 2006 290(6):C1633-1639. doi: 10.1152/ajpcell.00576.2005

Hein P, Frank M, Hoffmann C, Lohse MJ, Bünemann M. Dynamics of receptor/G protein coupling in living cells. EMBO J. 2005 24: 4106-4114. doi: 10.1038/sj.emboj.7600870

Frank M, Thümer L, Lohse MJ,  Bünemann M. G Protein activation without subunit dissociation depends on a Gα(i)-specific region. J Biol Chem. 2005 280: 24584-24590. doi: 10.1074/jbc.M414630200

Humrich J, Bermel C, Bünemann M, Harmark L, Frost R, Quitterer U, Lohse MJ. Phosducin-like protein regulates G-protein ßgamma folding by interaction with tailless complex polypeptide-1α: dephosphorylation or splicing of PhLP turns the switch toward regulation of Gßgamma folding. J Biol Chem. 2005 280: 20042-50. doi: 

Hoffmann C, Gaietta G, Bünemann M, Adams SR, Oberdorff-Maass S, Behr B,  Vilardaga JP, Tsien RY, Ellisman MH, Lohse MJ (2005). A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells. Nature Methods 2: 171-6.

Krasel C, Bünemann M,  Lorenz K, Lohse MJ (2005). Beta-arrestin binding to the ß2-adrenergic receptor requires both receptor phosphorylation and receptor activation. J Biol Chem. 280: 9528-35.

Nikolaev VO, Bünemann M, Hein L, Hannawacker A, Lohse MJ (2004). Novel single chain cAMP sensors for receptor-induced signal propagation. J Biol Chem. 279:37215-37218.  

Bünemann M, Frank M, Lohse MJ (2003). Gi protein activation in intact cells involves subunit rearrangement rather than dissociation. Proc Nat Acad Sci 100:16077-16082.

Lohse MJ, Vilardaga JP, Bünemann M (2003). Direct optical recording of intrinsic efficacy at a G protein-coupled receptor. Life Sciences; 74:397-404.

Lohse MJ, Vilardaga JP, Bünemann M (2003). Molecular mechanisms of receptor activation: real-time analysis by fluorescence resonance energy transfer. AutonAutacoid Pharmacol. 2003; 23: 231-3.

Vilardaga JP*, Bünemann M*, Krasel C, Castro M, Lohse MJ (2003). Measurement of the millisecond activation switch of G protein-coupled receptors in living cells. Nature Biotechnology; 21:807-812. * These authors contributed equally.

Hommers LG, Lohse MJ, Bünemann M (2003). Regulation of the inward rectifying properties of G-protein-activated inwardly rectifying K+ (GIRK) channels by G beta gamma subunits. J Biol Chem. 278:1037-1043.

Roseberry AG, Bünemann M, Elavunkal J, Hosey MM (2001). Agonist-dependent delivery of M-2 muscarinic acetylcholine receptors to the cell surface after pertussis toxin treatment. Molecular Pharmacology 59:1256-1268.

Liliom K, Sun GP, Bünemann M, Virag T, Nusser N, Baker DL, Wang DA, Fabian MJ, Brandts B, Bender K, Eickel A, Malik KU, Miller DD, Desiderio DM, Tigyi G, Pott L (2001). Sphingosylphosphocholine is a naturally occurring lipid mediator in blood plasma: a possible role in regulating cardiac function via sphingolipid receptors. Biochemical Journal; 355:189-197.

Bünemann M, Bücheler MM, Philipp M, Lohse MJ, Hein L (2001). Activation and deactivation kinetics of alpha(2A)- and alpha(2C)-adrenergic receptor-activated G protein-activated inwardly rectifying K⁺ channel currents. J Biol Chem. 276:47512-47517.

Gao TY, Cuadra AE, Ma H, Bünemann M, Gerhardstein BL, Cheng T, Ten Eick R, Hosey MM (2001). C-terminal fragments of the alpha(1C) (Ca(v)1.2) subunit associate with and regulate L-type calcium channels containing C-terminal-truncated alpha(1C) subunits. J Biol Chem.; 276:21089-21097.

Bünemann M, Hosey MM (2001). Novel signalling events mediated by muscarinic receptor subtypes. Life Sciences 68:2525-2533.

Wellner-Kienitz MC, Bender K, Meyer T, Bünemann M, Pott L (2000). Overexpressed A(1) adenosine receptors reduce activation of acetylcholine-sensitive K⁺ current by native muscarinic M-2 receptors in rat atrial myocytes. Circulation Research 86:643-648.

Lee KB, Ptasienski JA, Bünemann M, Hosey MM (2000). Acidic amino acids flanking phosphorylation sites in the M-2 muscarinic receptor regulate receptor phosphorylation, internalization, and interaction with arrestins. J Biol Chem.  275:35767-35777.

Gao TY, Bünemann M, Gerhardstein BL, Ma H, Hosey MM (2000). Role of the C terminus of the alpha(1C) (Ca(v)1.2) subunit in membrane targeting of cardiac L-type calcium channels. J Biol Chem.; 275:25436-25444.

Bünemann M, Meyer T, Pott L, Hosey M (2000). Novel inhibition of G beta gamma-activated potassium currents induced by M-2 muscarinic receptors via a pertussis toxin-insensitive pathway. J Biol Chem. 275:12537-12545.

Gerhardstein BL, Gao TY, Bünemann M, Puri TS, Adair A, Ma H, Hosey MM (2000). Proteolytic processing of the C terminus of the alpha(1C) subunit of L-type calcium channels and the role of a proline-rich domain in membrane tethering of proteolytic fragments. J Biol Chem. 275:8556-8563.

Liliom K, Bünemann M, Sun GP, Miller D, Desiderio DM, Brandts B, Bender K, Pott L, Nusser N, Tigyi G (2000). Sphingosylphosphorylcholine is a bona fide mediator regulating heart rate. Annals of the New York Academy of Sciences: Lysophospholipids and Eicosanoids in Biology and Pathophysiology; 905:308-310.

Hosey MM, Pals-Rylaarsdam R, Lee KB, Roseberry AG, Benovic JL, Gurevich VV, Bünemann M (1999). Molecular events associated with the regulation of signaling by M-2 muscarinic receptors. Life Sciences 64:363-368.

Bünemann M, Gerhardstein BL, Gao TY, Hosey MM (1999). Functional regulation of L-type calcium channels via protein kinase A-mediated phosphorylation of the beta(2) subunit. J Biol Chem.; 274:33851-33854.

Gilchrist A, Bünemann M, Li A, Hosey MM, Hamm HE (1999). A dominant-negative strategy for studying roles of G proteins in vivo. J Biol Chem.274:6610-6616.

Bünemann M, Hosey MM (1998). Regulators of G protein signaling (RGS) proteins constitutively activate G beta gamma-gated potassium channels. J Biol Chem. 273:31186-31190.

Bünemann M, Brandts B, Pott L (1997). In vivo downregulation of M-2 receptors revealed by measurement of muscarinic K+ current in cultured guinea-pig atrial myocytes. Journal of Physiology 501:549-554.

Bünemann M, Brandts B, Pott L (1996). Downregulation of muscarinic M(2) receptors linked to K+ current in cultured guinea-pig atrial myocytes. Journal of Physiology 494:351-362.

Brandts B, Bünemann M, Hluchy J, Sabin GV, Pott L (1997). Inhibition of muscarinic K+ current in guinea-pig atrial myocytes by PD 81,723, an allosteric enhancer of adenosine binding to A(1) receptors. British Journal of Pharmacology 121:1217-1223.

vanKoppen CJ, Heringdorf DMZ, Laser KT, Zhang CY, Jakobs KH, Bünemann M, Pott L (1996). Activation of a high affinity G(i) protein-coupled plasma membrane receptor by sphingosine-1-phosphate. Journal of Biological Chemistry 271:2082-2087.

Bünemann M, Liliom K, Brandts BK, Pott L, Tseng JL, Desiderio DM, Sun GP, Miller D, Tigyi G (1996). A novel membrane receptor with high affinity for lysosphingomyelin and sphingosine 1-phosphate in atrial myocytes. EMBO Journal; 15:5527-5534.

Bünemann M, Brandts B, Heringdorf DMZ, vanKoppen CJ, Jakobs KH, Pott L (1995). Activation of muscarinic K+ current in guinea-pig atrial myocytes by sphingosine-1-phosphate. Journal of Physiology 489:701-707.

Bünemann M, Pott L (1995). Down-Regulation of A(1) Adenosine Receptors Coupled to Muscarinic K+ Current in Cultured Guinea-Pig Atrial Myocytes. Journal of Physiology 482:81-92.

Bünemann M, Pott L (1993). Membrane-Delimited Activation of Muscarinic-K Current by an Albumin-Associated Factor in Guinea-Pig Atrial Myocytes. Pflügers Archiv-European Journal of Physiology; 425:329-334.

Banach K, Bünemann M, Hüser J, Pott L (1993). Serum Contains a Potent Factor That Decreases Beta-Adrenergic Receptor-Stimulated L-Type Ca-2+ Current in Cardiac Myocytes. Pflügers Archiv-European Journal of Physiology 423:245-250.

Übersichtsartikel:

Vilardaga JP, Bünemann M, Feinstein TN, Lambert N, Nikolaev VO, Engelhardt S,

Lohse MJ, Hoffmann C. (2009) GPCR and G proteins: drug efficacy and activation in live

cells. Mol Endocrinol. 5:590-9.

Hein P, Bünemann M. Coupling mode of receptors and G proteins. (2008) Naunyn

SchmiedebergsArch Pharmacol. 2009 May;379(5):435-43.

Hoffmann C, Zürn A, Bünemann M Lohse MJ (2008) Conformational changes in G-protein-coupled receptors-the quest for functionally selective conformations is open. Br J Pharmacol. 153: S358-66

Lohse MJ, Nikolaev VO, Hein P, Hoffmann C, Vilardaga JP, Bünemann M (2008). Optical techniques to analyze real-time activation and signaling of G-protein-coupled receptors. Trends Pharmacol Sci 8: 8

Lohse MJ, Hein P, Hoffmann C, Nikolaev VO, Vilardaga JP Bünemann M (2008) Kinetics of G-protein-coupled receptor signals in intact cells. Br J Pharmacol. 153:S125-32

Lohse MJ, Bünemann M, Hoffmann C, Vilardaga JP, Nikolaev VO (2007). Monitoring receptor signaling by intramolecular FRET  Curr Opin Pharmacol (5):547-53.

Lohse MJ, Hoffmann C, Nikolaev VO, Vilardaga JP, Bünemann M (2007). Kinetic analysis of G protein-coupled receptor signaling using fluorescence resonance energy transfer in living cells. Adv Protein Chem 74:167-88.

Krasel C, Vilardaga JP, Bünemann M, Lohse MJ. (2004) Kinetics of G-protein-coupled receptor signalling and desensitization. Biochemical Society Transactions  32(Pt 6):1029-31.

Bünemann M, Lee KB, Pals-Rylaarsdam R, Roseberry AG, Hosey MM.(1999) Desensitization of G-protein-coupled receptors in the cardiovascular system. Annual Review of Physiology  61:169-192.

Bünemann M, Hosey MM.(1999) G-protein coupled receptor kinases as modulators of G-protein signalling. Journal of Physiology 517:5-23.

Patente:

1. Vilardaga JP, Hoffmann C, Bünemann M, Lohse MJ (2004, appl.) Millisecond activation switch for seven-transmembrane proteins. European Patent Office WO200405733A1, EP1687441

2. Nikolaev VO, Bünemann M, Lohse MJ (2005, appl.) Means and methods for optical determination of cAMP in vitro and in vivo. European Patent Office WO2005052186, EP1536020