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Standort B
Zeiss LSM980 with extra Rapp UV-Box (CLSM)
· Axio Observer, inverse, motorized XY, 10 nm focus steps, Z-piezo with 500 µm range, motorized filter ring, condensor with shutter, black incubator box
· Solid state laser 405nm, 488nm, 514nm, 561nm, 594nm, 639nm
· Airyscan 2 array detector, Spectral GaAsP detector, 2x photomultiplier detector
· Photoncounting mode
· Autofocus, focus control in longterm imaging
· Second independent scan module (Rapp UGA-42 caliburn)for illumination via epiport
· UV laser box: 355/42 nm (pulsed), 375/70 nm (continuous wave)
· large system table NLO table, 1.5x1.8m
· second computer, linked to system for real-time data transfer and processing
Inhalt ausklappen Inhalt einklappen LSM980 Objectives
· C-Apochromat 40x/1.2 W
· Plan-Apochromat 63x/1.4 oil
· LCI Plan-Apochromat 63x/1.2 multi immersion
Inhalt ausklappen Inhalt einklappen LSM980 special applications
o Recording based highresolution imaging down to 120 nm lateral
o Multiplex imaging (up to eight lines simultaneously)
o FRAP
o fluctuation analysis and FCS
o Laser ablation with pulsed UV laser, junctions cuts, wounding, organelle ablation, microsurgery
o UV uncaging with 375 nm UV laser, e.g. NP-EGTA für Ca2+ uncaging
o realtime processing and data transfer to second computer
o longterm (multiple hours) time lapse imaging with readjusted axial focus
o tiling of large objects (mm scale)
Zeiss AxioObserver (Epifluorescence)
· AxioObserver Z1, inverted, differential interference contrast, phase contrast, motorized stage with joystick
· Detectors: AxiocamMR, emCCD (photometrics evolve 512), ApoTome
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· 10x
· 20x
· 40x
· 63x/oil
· 100x/oil
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o Optical sectioning with ApoTome
o Simultaneous time lapse recordings (DIC, wide field) with tilling and position module
Zeiss AxioPlan (Epifluorescence)
· Axioplan 2, upright, differential interference contrast, phase contrast, darkfield optics
· Detectors AxiocamMR, ApoTomeI
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· 10x
· 20x
· 40x/oil
· 63x/oil
· 63x/PH/oil
· 100x/oil
Inhalt ausklappen Inhalt einklappen Axioplan special applications
o Optical sectioning with ApoTome
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