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 Anaerobic indoleacetate metabolism

We have identified a complex pathway of anaerobic indoleacetate (auxin) degradation, which involves activation to the CoA-thioester, hydroxylation via a molybdoenzyme, ATP-dependent ring hydrolysis, a coenzyme B12-dependent rearrangement reaction and further novel steps. We are interested in the biochemical and structural characterisation of the enzymes of this pathway and their application in synthetic pathways.

Fig. 6: Pathway of anaerobic indoleacetate metabolism. © Schühle K., Nies J. & Heider J. (2016) An indoleactate-CoA ligase and a phenylsuccinate CoA-transferase involved in anaerobic indoleacteate metabolism. Environ. Microbiol. 18, 3120-3132