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NanoAnalyzer - NanoFCM
![](https://www.uni-marburg.de/en/fb20/departments/zti/institute-for-tumor-immunology/ev-core-facility/equipment-1/nanofcm.jpg/@@images/image/_unimr_preview)
The Nanoanaylzer is a robust, fast, and easy-to-use nano-flow cytometry analyzer for the measurement of the concentration and size of biological and synthetic nanoparticles (>40 nm). By combining size determination by scattering, based on the dedicated nano-flow system operating at low flow rates and low sheath flows, and the ability to detect particles phenotypically with up to 2 built-in lasers, it can partially replace other analytical methods. Of particular note are the very good resolution in the 40-200 nm range and the combination of scattering and fluorescence signal, which minimizes the detection of false positives.
Combining all these characteristics, the nano-flow cytometry provides a versatile approach for analyzing and profiling EVs from different sources such as cell culture supernatants and various body fluids.
![](https://www.uni-marburg.de/en/fb20/departments/zti/institute-for-tumor-immunology/ev-core-facility/equipment-1/nfcm_scatter.jpg/@@images/image/_unimr_preview)
After labeling with fluorescent antibodies, the fluorescence signals of EVs can be detected concurrently with side scatter.
Available configuration:
Blue 488nm and Red 640nm
Detector |
Dichroic mirror |
Bandpass filter |
Channel |
SPCM-1 | 488/10 | SSC | |
SPCM-2 | DicF495 | 525/40 | FITC/GFP/AF488 |
SPCM-3 | DicF555 | 670/30 | PE-Cy5/PerCP/AF647/APC |
optional | 580/40 | PE/AF555/SYTO82 | |
optional | 710/40 | PerCP-Cy5.5/PE-AF680 |