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A04 - Sjaak Philipsen/Thamar van Dijk:
Repurposing CRISPR/Cas9 for targeted demethylation of the gamma-globin promoters
Reactivation of fetal globin expression is clinically relevant for beta-hemoglobinopathy patients. We will determine whether DNA methylation of the fetal gamma-globin gene promoters is involved in their suppression in adult erythropoiesis. To achieve local DNA demethylation, we will take advantage of CRISPR/Cas9 technology to direct TET methylcytosine dioxygenases to the gamma-globin gene promoters. In addition, we will study the interplay of DNA methylation with putative fetal globin modulating pharmacological compounds, focussing on inhibitors of protein arginine methyltransferases.
Email: j.philipsen@erasmusmc.nl
Phone: +31 10-7044282
Email: t.vandijk@erasmusmc.nl
Phone: +31 10-7044282
